Color Standards

AbLead employs intuitive coloring schemes to represent sequence properties and structural features, enabling rapid visual analysis of developability and engineering targets.

ABHAND

The ABHAND scheme classifies residues by their physicochemical properties. This allows engineers to quickly spot charge distributions, hydrophobic patches, and potential liabilities.

Group	Color	Hex	Residue Properties
Acidic	Red	`#FF0000`	Negatively charged (D, E)
Basic	Blue	`#0000FF`	Positively charged (K, R, H)
Hydrophobic	Green	`#00FF00`	Non-polar, aliphatic (A, L, I, M, C, V, P)
Aromatic	Magenta	`#FF00FF`	Large aromatic rings (F, W, Y)
Neutral	Yellow	`#FFFF00`	Uncharged polar (N, Q, S, T, B, Z)
Deletion	Black	`#000000`	Glycine (G), Stop (*), Unknown (X)
Gaps	White	`#FFFFFF`	Deletions or gaps in alignment (-)

Region Coloring

Structural regions are color-coded to distinguish between the stable Framework (FMWK) and the variable Complementarity-Determining Regions (CDRs).

Framework Regions

Used to provide structural support. Heavy chain frameworks are darker to provide visual contrast.

Chain	Color	Hex
Light Chain FMWK	Silver	`#C0C0C0`
Heavy Chain FMWK	Dim Gray	`#696969`

Complementarity-Determining Regions (CDRs)

Key regions for antigen binding. Light and heavy chain CDRs are differentiated by shade.

Region	Light Chain	Hex	Heavy Chain	Hex
CDR1	Dodger Blue	`#1E90FF`	Blue	`#0000FF`
CDR2	Medium Purple	`#9370DB`	Dark Orchid	`#9932CC`
CDR3	Cyan	`#00FFFF`	Dark Cyan	`#008B8B`

Liability Colors

The liability coloring scheme is used in the Liabilities view and SVL exports to highlight specific chemical and structural risks. These colors are distinct from the general severity colors to allow for identification of the specific liability type.

Liability	Color	Hex
AbLang	Red	`#FF0000`
AbLang2	Red (AbLang2)	`#E60000`
IgBert	Red (IgBert)	`#CC0000`
Disrupted CDR3 Salt Bridge	Red/Coral	`#FF5C5C`
Unusual/Missing Cysteine	Yellow	`#FFFF00`
Deamidation (High)	Pale Green	`#90EE90`
Deamidation (Medium)	Medium Green	`#73BE73`
Deamidation (Low)	Dark Green	`#568F56`
Isomerization (High)	Magenta	`#FF00FF`
N-glycosylation (High)	Light Salmon	`#FFA07A`
Met Oxidation (Low)	Pink	`#FFA8BB`
Trp Oxidation (Medium)	Firebrick	`#B22222`
Hydrolysis (Medium)	Muted Green	`#90BB90`
Fragmentation (High)	Purple	`#CC00CC`
Fragmentation (Medium)	Dark Purple	`#A300A3`

Severity Colors

Severity colors are used to flag developability risks, such as chemical liabilities or poor humanness and stability scores.

Severity	Color	Hex	Description
High	Red	`#CF4A3C`	Significant liability or extreme score deviation.
Medium	Orange	`#E29848`	Potential liability or moderate score deviation.
Low	Yellow	`#F8D548`	Minor liability or slight score deviation.
Good	Green	`#88B46C`	Ideal range or no liabilities detected.
Baseline	Tan	`#E6DEBC`	Neutral status or baseline reference.

NOTE: Some specialized tools, such as the Humanization dashboard, may use these same colors to represent different metrics (e.g., status relative to germline). Always refer to the legend within the specific tool's sidebar.

Engineering and Observation Indicators

Residue-level indicators (small dots in the upper-right corner of a residue cell) provide real-time visual synchronization between the Engineering database and the various analysis tools (Alignment, PFA, Liabilities, and Humanness).

Indicator Type	Visual	Hex	Description
Mutation Design	Eggshell	`#F0EAD6`	A mutation or deletion design exists for this residue in the Engineering tool.
Observation	Orange	`#FF7F00`	A sequence observation (e.g., experimental result) has been recorded for this residue.
Both	Powder Blue	`#9EBFF0`	Both an Engineering design and an Observation exist for this specific residue.

TIP: These indicators are reactive across all tabs. Clicking a residue with an indicator in a tool which allows residue-click editing (e.g. Alignment tool) will show these on the specific residue.