Color Standards
AbLead employs intuitive coloring schemes to represent sequence properties and structural features, enabling rapid visual analysis of developability and engineering targets.
ABHAND
The ABHAND scheme classifies residues by their physicochemical properties. This allows engineers to quickly spot charge distributions, hydrophobic patches, and potential liabilities.
| Group | Color | Hex | Residue Properties |
|---|---|---|---|
| Acidic | Red |
#FF0000 |
Negatively charged (D, E) |
| Basic | Blue |
#0000FF |
Positively charged (K, R, H) |
| Hydrophobic | Green |
#00FF00 |
Non-polar, aliphatic (A, L, I, M, C, V, P) |
| Aromatic | Magenta |
#FF00FF |
Large aromatic rings (F, W, Y) |
| Neutral | Yellow |
#FFFF00 |
Uncharged polar (N, Q, S, T, B, Z) |
| Deletion | Black |
#000000 |
Glycine (G), Stop (*), Unknown (X) |
| Gaps | White | #FFFFFF |
Deletions or gaps in alignment (-) |
Region Coloring
Structural regions are color-coded to distinguish between the stable Framework (FMWK) and the variable Complementarity-Determining Regions (CDRs).
Framework Regions
Used to provide structural support. Heavy chain frameworks are darker to provide visual contrast.
| Chain | Color | Hex |
|---|---|---|
| Light Chain FMWK | Silver |
#C0C0C0 |
| Heavy Chain FMWK | Dim Gray |
#696969 |
Complementarity-Determining Regions (CDRs)
Key regions for antigen binding. Light and heavy chain CDRs are differentiated by shade.
| Region | Light Chain | Hex | Heavy Chain | Hex |
|---|---|---|---|---|
| CDR1 | Dodger Blue |
#1E90FF |
Blue |
#0000FF |
| CDR2 | Medium Purple |
#9370DB |
Dark Orchid |
#9932CC |
| CDR3 | Cyan |
#00FFFF |
Dark Cyan |
#008B8B |
Severity Colors
Severity colors are used to flag developability risks, such as chemical liabilities or poor humanness and stability scores.
| Severity | Color | Hex | Description |
|---|---|---|---|
| High | Red |
#CF4A3C |
Significant liability or extreme score deviation. |
| Medium | Orange |
#E29848 |
Potential liability or moderate score deviation. |
| Low | Yellow |
#F8D548 |
Minor liability or slight score deviation. |
| Good | Green |
#88B46C |
Ideal range or no liabilities detected. |
| Baseline | Tan |
#E6DEBC |
Neutral status or baseline reference. |
[!NOTE] Some specialized tools, such as the Humanization dashboard, may use these same colors to represent different metrics (e.g., status relative to germline). Always refer to the legend within the specific tool's sidebar.