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Bulk Humanize...

[!WARNING] This method is not meant for lead humanization. It is to test a simple humanization on a large number of candidates. Use a more thoughtful approach for a final lead.

To humanize multiple antibodies in a single batch, select the desired entries in the Project View, open the Edit menu, and select Bulk Humanize....

After choosing a target species, the tool will automatically:

  • Apply standard Honegger exclusions (plus VHH-specific solubility exclusions at heavy chain positions 42, 49, 50, and 52 if the entry is a VHH).

  • Determine the closest V/J germlines using the Modifiable Residues Identity method (matching sequence identity only on positions allowed to mutate).

  • Generate humanized sequences and register them directly back into the project database under <Parent>_Hum names (bypassing the Engineering workspace).

  • Automatically enqueue a targeted background re-analysis run that processes only the newly created entries.